Measurement of human proinsulin by an indirect two-site immunoradiometric assay
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چکیده
منابع مشابه
Measurement of intact human parathyrin by an extracting two-site immunoradiometric assay.
This is an immunoradiometric assay of intact human parathyrin, hPTH(1-84). One antibody, directed against the N-terminal part of the hormone, was produced in goats and conjugated covalently to cellulose particles. hPTH(1-84) and the N-terminal fragments were extracted from EDTA-treated plasma by these particles and thus concentrated. Another antibody, against synthetic hPTH(53-84), was raised i...
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We developed a two-site immunoradiometric assay (IRMA) of salmon calcitonin (SCT) that detects intact SCT(1-32) and not peptide fragments of the hormone. This was accomplished by using monoclonal antibodies prepared against the peptide fragments SCT(1-11) and SCT(11-32). Two antibodies with specificity for each of the peptides were purified from their respective ascites and evaluated in a two-s...
متن کاملTwo-site immunoradiometric assay (IRMA): detection, efficiency, and procedural modifications.
A 2-site immunoradiometric assay (IRMA) has been done using Anopheles stephensi fed on Plasmodium vivax blood through parafilm to ascertain at what time in the sporogonic cycle circumsporozoite (CS) antigen can be detected, and to detect CS antigen in mosquitoes squashed on filter paper (FP) and cellulose acetate membrane (CAM). The CS antigen was detectable only in mature oocysts, a day prior ...
متن کاملImmunoradiometric assay of human proinsulin and partially processed proinsulin with use of monoclonal antibody and streptavidin-biotin labeling.
The sensitive and specific immunoradiometric assay is described for human proinsulin and its intermediate peptides (65-66 split and 32-33 split proinsulin). We developed a monoclonal antibody-based two-site immunoradiometric assay with use of streptavidin-biotin labeling. The detection limits of the assays lie in the range of 0.5-2.0 pM. In the proinsulin assay proinsulin cross-reacted 66% with...
متن کاملAn automated immunoradiometric assay for human thyrotropin.
In this two-site immunoradiometric assay for thyrotropin, developed for use in the "Kemtek 3000" automated radioimmunoassay system, commercially available monoclonal antibody to thyrotropin is labeled with 125I, and the solid-phase antibody is an IgG fraction of sheep antiserum to thyrotropin, covalently coupled to reprecipitated aminocellulose. There are two incubations, totalling 3 h, the sen...
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ژورنال
عنوان ژورنال: Diabetologia
سال: 1979
ISSN: 0012-186X,1432-0428
DOI: 10.1007/bf01235859